biocomp gradient master 108 (Biocomp Instruments Inc)

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Biocomp Instruments Inc biocomp gradient master 108
Biocomp Gradient Master 108, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biocomp+gradient+master+108/biocomp+gradient+master/pmc10907855-91-6-10
Average 90 stars, based on 1 article reviews

biocomp gradient master 108 - by Bioz Stars, 2026-09

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Article Title: Structural basis of transcription reduction by a promoter-proximal +1 nucleosome
Article Snippet: The gradient was prepared from 15% to 40% sucrose in a buffer with 20 mM HEPES pH 7.5, 100 mM KCl, 2.5% glycerol, 2 mM MgCl 2 , 1 mM TCEP, via a BioComp Gradient Master 108 (BioComp Instruments).

Article Title: Recruitment of the 40S ribosomal subunit by the West Nile virus 3′ UTR promotes the cross-talk between the viral genomic ends for translation regulation
Article Snippet: Sucrose gradients were prepared using the BioComp Gradient Master 108 (BioComp Instruments, Fredericton, NB, Canada), following manufacturer's instructions.

Article Title: Structure of the human Mediator-RNA polymerase II pre-initiation complex.
Article Snippet: Mediator is a conserved coactivator complex that enables the regulated initiation of transcription at eukaryotic genes.. Mediator is recruited by transcriptional activators and binds the pre-initiation complex (PIC) to stimulate the phosphorylation of RNA polymerase II (Pol II) and promoter escape.. Here we prepare a recombinant version of human Mediator, reconstitute a 50-subunit Mediator–PIC complex and determine the structure of the complex by cryo-electron microscopy.

Article Title: Structural basis of transcription reduction by a promoter-proximal +1 nucleosome.
Article Snippet: The gradient was prepared from 15% to 40% sucrose in a buffer with 20 mM HEPES pH 7.5, 100 mM KCl, 2.5% glycerol, 2 mM MgCl2, 1 mM TCEP, via a BioComp Gradient Master 108 (BioComp Instruments).

Article Title: Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain
Article Snippet: The gradient was prepared using a 15% sucrose solution [15% (w/v) sucrose, 100 mM KCl, 25 mM K·4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (HEPES) pH 7.5, 2 mM MgCl 2 , 2.5% glycerol (v/v), 1 mM tris(2-carboxyethyl)phosphine (TCEP)] and a 40% sucrose solution [40% (w/v) sucrose, 100 mM KCl, 25 mM K·HEPES pH 7.5, 2 mM MgCl 2 , 2.5% glycerol (v/v), 1 mM TCEP, 0.1% (v/v) glutaraldehyde] with a BioComp Gradient Master 108 (BioComp Instruments).

Article Title: Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain.
Article Snippet: The gradient was prepared using a 15% sucrose solution [15% (w/v) sucrose, 100 mM KCl, 25 mM K·4- (2- hydroxyethyl)- 1- piperazineethanesulfonic acid) (HEPES) pH 7.5, 2 mM MgCl2, 2.5% glycerol (v/v), 1 mM tris(2- carboxyethyl) phosphine (TCEP)] and a 40% sucrose solution [40% (w/v) sucrose, 100 mM KCl, 25 mM K·HEPES pH 7.5, 2 mM MgCl2, 2.5% glycerol (v/v), 1 mM TCEP, 0.1% (v/v) glutaraldehyde] with a BioComp Gradient Master 108 (BioComp Instruments).

Article Title: Structure of human Mediator-RNA polymerase II transcription pre-initiation complex
Article Snippet: The gradient was prepared using a BioComp Gradient Master 108 (BioComp Instruments) by mixing a 15% sucrose solution (15% sucrose, 25 mM HEPES pH 7.6, 100 mM KCl, 5 mM MgCl 2 , 5% glycerol (v/v) and 3 mM TCEP) and a 40% sucrose solution (40% sucrose, 25 mM HEPES pH 7.6, 100 mM KCl, 5 mM MgCl2, 5% glycerol (v/v) and 3 mM TCEP) supplemented with 0.25% of glutaraldehyde (v/v) crosslinker.

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Article Title: Structures of mammalian RNA polymerase II pre-initiation complexes.
Article Snippet: The initiation of transcription is a focal point for the regulation of gene activity during mammalian cell differentiation and development.. To initiate transcription, RNA polymerase II (Pol II) assembles with general transcription factors into a pre-initiation complex (PIC) that opens promoter DNA.. Previous work provided the molecular architecture of the yeast and human PIC and a topological model for DNA opening by the general transcription factor TFIIH.

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